骨髓基质细胞移植促进脑缺血大鼠海马巢蛋白的表达

Transplantation of bone marrow stromal cells upregulates the expression of Nestin in rat hippocampus after cerebral ischemia

目的:探讨移植骨髓基质细胞(BMSCs)对永久性局灶性脑缺血大鼠海马巢蛋白表达的影响。方法:制备永久性局灶性脑缺血大鼠模型,分为PBS对照组和治疗组,再将每组动物随机均分为脑缺血后7d和14d亚组。PBS对照组在脑缺血后1d注射PBS,治疗组在脑缺血后第1日移植BMSCs。Zausinger六分法检测神经功能恢复情况;免疫组织化学方法检测大鼠海马的巢蛋白表达。结果:在脑缺血后第7日和第14日两个时相点,治疗组的神经功能评分均较高,与PBS对照组之间存在显著性差异;齿状回的颗粒细胞下层、颗粒细胞层和海马锥体细胞层均有巢蛋白表达,在脑缺血后第14日,治疗组的大鼠患侧海马的巢蛋白阳性细胞较对照组表达高,存在显著性差异。结论:BMSCs可以改善脑缺血大鼠的神经功能;促进海马内神经干细胞或反应性星形胶质细胞增殖可能为其修复缺血脑损伤的机制之一。

Objective： To explore the effect of transplantation of bone marrow stromal cells on the expression of Nestin in rat hippocampus after permanent focal cerebral ischemia. Methods： The rats were induced to permanent focal cerebral ischemia models with middle cerebral artery occlusion （MCAO）. Experimental groups divided into 2 groups： intravenous infusion of 1 ml PBS at 24 hours after MCAO, and intravenous infusion of 2×10^6 BMSCs in PBS at 24 hours after MCAO, and each group has 7 and 14 days subgroup. Neurological functions were detected using Zausinger evaluation. Immunohistochemistry was used to detect the Nestin expression in the hippocampus. Results： At 7 days or 14 days after MACO, neurological functional scores of BMSCs-treated group were higher than another group （P〈 0. 05）. Nestirrpositive cells were found in the subgranular zone （SGZ） and granular layer of dentate gyms and pyramidal cell layer of the hippocampus. At 14 days after ischemia, Nestirrpositive cells in hippocampus of ipsilateral ischemia of BMSCs-treated group were more than those in the control group （P 〈 0. 05. ） Conclusion： BMSCs can improve the neurological function of the rats with permanent focal cerebral ischemia. Enhancing the proliferation of neural stem cells and reactive astrocyte in the hippocarnpus may contribute to the therapy for cerebral ischemia.