摘要
目的建立用RP-HPLC法测定白芍总苷微孔渗透泵控释片中芍药苷含量的方法。方法采用Diamonsil C_(18)色谱柱(5μm,250mm×4.6mm),以甲醇-水-磷酸(体积比33:67:0.2)为流动相,检测波长230nm。结果芍药苷质量浓度在20~100μg/ mL范围内线性关系良好(r=0.9995),平均加样回收率100.6%(n=6)。结论本法准确、简便、专属性强,可用于白芍总苷微孔渗透泵控释片的质量控制。
Objective To establish a RP-HPL method for determination of paeoniflorin in controlled porosity osmotic pump tablets of total glucosides of paeony.Methods A Diamonsil C18 colum (5μm,250 mm×4.6 mm) was used by using methanol-water-phosphate (33:67:0.2),as mobile phase.The detection wavelength was 230 nm.Results The linear range was 20~100μg/mL (r=0.9995). Conclusion The method is convenient,accurate and specific,which could be used for the quality control of total glucosides of paeony controlled porosity osmotic pump tablets.
出处
《广东药学院学报》
CAS
2007年第6期633-634,657,共3页
Academic Journal of Guangdong College of Pharmacy
关键词
高效液相色谱法
白芍总苷
芍药苷
微孔渗透泵控释片
paeoniflorin
total glucosides of paeony
controlled porosity osmotic pump tablets
HPLC