表没食子儿茶素没食子酸酯微囊含量测定方法学研究

HPLC determination of epigallocatechin gallate microcapsules

目的建立测定表没食子儿茶素没食子酸酯微囊含量的方法。方法采用HPLC法,Hypersil ODS柱(250mm×4.6mm,5μm)为固定相,流动相为0.1%磷酸溶液、乙腈线性梯度洗脱(体积比90:10→50:50);检测波长为273nm;流速为1.0mL/ min;柱温28℃。结果本方法在7.76～776μg/mL呈良好线性关系(r=0.9999),进样重现性RSD=0.75%(n=5),平均回收率为99.53%,日内精密度RSD为0.87%(n=5)。结论本方法简便、快速,结果准确可靠,可用于表没食子儿茶素没食子酸酯微囊含量测定。

Objective To establish an external standard HPLC method for the determination of epigallocatechin gallate （EGCG） microcapsules.Methods Hypersil ODS column was used with 0.1% H3PO4 and acetonitrile as mobile phase,detection wavelength：273 nm;flow rate 1.0 mL/min;and column temperature 28℃.Results The linear range of the method was 7.76～776μg/mL （r= 0.9999）,RSD=0.75% （n=5）,and the average recovery was 99.53%.RSD of within-day precision was 0.87% （n=5）.Conclusions The method is simple,fast and accurate for the analysis of EGCG microcapsules.