人巨噬细胞金属弹性蛋白酶在胃癌细胞和组织中的表达及临床意义

The expression of human macrophage metalloelastase mRNA in gastric cancer cell lines and tissues and its clinical significance

目的测定人巨噬细胞金属弹性蛋白酶(HME)在胃癌细胞及胃癌组织中的表达,评价其在胃癌进展中的作用。方法采用实时荧光PCR测定胃癌细胞及胃癌组织中HME mRNA表达水平,并对胃癌组织中HME mRNA表达与患者临床病理特征及预后的关系进行统计分析。结果MGC-803、SGC-7901和AGS胃癌细胞株均有HME mRNA的表达。胃癌组织、癌旁组织和正常组织的HME mRNA表达阳性率分别为31.03%、25.87%和18.97%,阳性结果拷贝数对数值分别为6.06±0.76、3.53±0.85和1.88±0.82,胃癌组织HME tuRNA表达阳性率、HME mRNA表达量均显著高于正常组织(P<0.05)。HME mRNA表达与肿瘤的位置、大小、浸润深度、耐药基因topⅡ和PG的表达无关,胃癌组织HME mRNA阳性患者淋巴结转移发生率低于阴性者、GST基因表达阳性率低于阴性者、2年生存率高于阴性者。结论胃癌组织中HME mRNA表达阳性与胃癌转移潜能降低有关,可能是预后良好的标志。

Objective To make measurement of the expression of HME mRNA in both gastric cancer cell lines and tissues and evaluate its role in development of gastric cancer. Methods The HME mRNA expression in 3 gastric cancer cell lines and tissues was detected by real-time fluorescent quantitative PCR. Results All three gastric cancer cell lines expressed HME mRNA, and the HME mRNA expression level in gastric cancer tissues was higher than that in normal tissues （ P 〈 0. 05 ）. The rate of lymph node metastasis of HME mRNA positive cases was lower than that of HME mRNA negative ones （P 〈 0.05）. Expression of drug resistance gene GST of HME mRNA positive cases was lower than that of HME mRNA negative ones （P 〈 0.05）. The two-year survival rate of HME mRNA positive cases was higher than that of HME mRNA negative ones （P 〈0.05 ）. There was no correlation between the expression of HME gene and the tumor location, size, depth of invasion, degree of malignancy, expression of drug resistance gene top Ⅱ and PG. Conclusion The expression of HME gene in gastric cancers may be related with lower possibility of metastasis and predict a better prognosis.