蛋白激酶C抑制剂对吸烟大鼠脑血管内皮细胞细胞间粘附分子1表达的影响

Effects of Protein Kinase C Inhibitor on the Expression of Intercellular Adhesion Molecule-1 Protein and mRNA on Cerebrovascularendothelial Cells in Smoking Rats

目的探讨蛋白激酶C抑制剂对吸烟大鼠脑血管内皮细胞细胞间粘附分子1蛋白和mRNA表达的影响。方法无脑梗死大鼠18只,随机分为不吸烟组6只、吸烟组6只和吸烟蛋白激酶C抑制剂组6只。脑梗死大鼠48只,随机分为脑梗死组24只和蛋白激酶C抑制剂组24只,分别于梗死后2、6、12及24h干预,每个时间点6只。采用免疫组织化学法和原位杂交法分别测定细胞间粘附分子1蛋白和mRNA。结果吸烟大鼠脑血管内皮细胞细胞间粘附分子1蛋白和mRNA均有表达,吸烟蛋白激酶C抑制剂组脑血管内皮细胞细胞间粘附分子1蛋白和mRNA的表达明显低于吸烟组(P<0.05)。蛋白激酶C抑制剂组细胞间粘附分子1蛋白和mRNA的表达均低于对应时间点脑梗死组(P<0.05),且梗死后2h蛋白激酶C抑制剂组细胞间粘附分子1蛋白和mRNA的表达明显低于其他时间点组。结论蛋白激酶C抑制剂可阻断吸烟大鼠脑血管内皮细胞细胞间粘附分子1蛋白和mRNA表达,并且早期用药效果可能更好。

Aim To investigate the effect of protein kinase C inhibitor on expression of intercellular adhesion molecule- 1 protein and mRNA in smoking rats. Methods The expression of intercellular adhesion molecule- 1 protein and mRNA were abserved by immunohistochemistry and in situ hybrizition. 18 non-infarcted rats were randomly divided into three groups including 6 non-smoking rats, 6 no medicine treated smoking rats and 6 protein kinase C inhibitor treated smoking rats. 48 infarcted rats were randomly divided into two groups including 24 no medicine treated rats and 24 protein kinase C inhibitor treated rats. 2 hours, 6 hours, 12 hours or 24 hours after infarction the medicine were given, 6 rots in each time points. Retails Compared with no medicine groups, the expression of intereelhlar adhesion mulecule-1 and intereellular adhesion molecule-1 mRNA were decreased with corresponding indentical time points in protein kinase C inhibitor groups, which showed statistical difference （P 〈 0.05）. Conclusions Protein kinase C inhibitor could reduce the expression of intercellular adhesion molecule-1 and intercellular adhesion molecule-1 mRNA in smoking rats cerebrovascularendothelial cells, more effect will be produced. the medicine were given more early.