老年人血浆脂蛋白相关磷脂酶A2活性与其基因型、性别和年龄的关系

Correlations Between Plasma Lipoprotein-Associated Phospholipase A2 Activity And Genotype,Sex and Age

目的研究血浆脂蛋白相关磷脂酶A2活性与脂蛋白相关磷脂酶A2基因、性别和年龄等因素的关系。方法应用聚合酶链反应技术和分光光度法,检测1867例50岁以上老年人的脂蛋白相关磷脂酶A2基因G994T的多态性及血浆脂蛋白相关磷脂酶A2活性。结果不同的脂蛋白相关磷脂酶A2基因型血浆脂蛋白相关磷脂酶A2活性差异存在显著(P<0.001),GG基因型活性最高,GT次之,TT最低;GG和GT基因型中,男性血浆脂蛋白相关磷脂酶A2活性显著高于女性(P<0.001),吸烟者酶活性显著高于不吸烟者(GG与GT基因型中分别为P<0.01和P<0.05);在GG基因型中,男性脂蛋白相关磷脂酶A2活性随年龄升高,在GT基因型中随年龄降低。结论血浆脂蛋白相关磷脂酶A2活性与基因型密切相关;男性脂蛋白相关磷脂酶A2活性高于女性,本研究中这种差异与吸烟有关;GG基因型中男性活性增高可能是一种对随年龄增长的血小板活化因子或氧化磷脂的应激反应的代偿机制,而这种代偿机制在GT中不明显。

Aim To evaluate effects of the G994T,sex,and age on the lipoprotein-Associated phospholipase A2（Lp-PLA2） activity.Methods Genotyping of the G994T was done by the allele-specific PCR.The Lp-PLA2 activity was measured using a commercial kit by the spectrophotometric method.Results The Lp-PLA2 levels were significantly higher in GG subpopulation,as compared to GT population and TT population.The Lp-PLA2 activity was correlated with sex.We found that,in men with the GG genotype,enzyme activity increased significandy with age. In contrast, plasma Lp-PLA2 activity in men with the GT genotype did not significantly increase with age. Conclusions The G994T was strongly correlated with the enzyme activity. The Lp-PLA2 activity in men was higher than that in women. Plasma PAF-AH activity may increase in response to stresses induced by PAF and： or oxidized phospholipids. This compensatory response may be attenuated or not evident in individuals with the m allele.