饮用纯净水协同食物低镁对围产期仔鼠学习与记忆能力的影响

Synergistic Effect of Purified Water and Magnesium Deficiency Food on Learning and Memory of Offspring of Rats

目的观察并比较食物中矿物元素含量正常(AIN-93G)及食物低镁条件下,饮用纯净水对子代神经行为发育的影响及食物中镁的协同作用。方法用正常饮食(镁含量为正常需要量,0.4g/kg)正常饮水[溶解性总固体为175mg/L]、正常饮食纯净水(TDS为1.16mg/L)、低镁饮食(镁含量为需要量的50%,0.2g/kg)正常饮水、低镁饮食纯净水饲喂断乳后的亲代SD大鼠(共78d)及其仔鼠(共35d),用Morris水迷宫检测仔鼠(28日龄)的学习记忆能力。结果在定位航行实验(反应学习能力)中,训练前期(前3d),与正常饮食正常饮水组相比较,低镁饮食正常饮水组仔鼠的逃逸潜伏期未出现显著性差异,而正常饮食纯净水组和低镁饮食纯净水组仔鼠的逃逸潜伏期均显著性延长(P<0.05),此趋势在训练后期仍然保持(后2d),但各组间的差异无统计学意义。随后的空间探索实验(反应记忆能力)及可视平台实验中,各组间差异未显示统计学意义。结论围产期饮用纯净水可能迟滞仔鼠早期学习能力形成,而食物中镁元素含量不足时,纯净水迟滞学习能力的作用更明显。

Objective To study the effect of purified water on the neurobehavioral development of the offspring and the synergistic effect of the magnesium deficiency in food. Methods SD rats were divided into four groups, control food plus control water （control group）, control food plus purified water, magnesium deficiency food plus control water, magnesium deficiency plus purified water, from five weeks of age of the F0 generation to five weeks of the F1 generation respectively. Morris water maze （MWM） was used to test the spatial learning and memory ability of offspring of rats. Results In the early training days of the place navigation, control group offspring of rats showed a shorter swimming time than the other groups. There were no significant differences among all experimental groups in the later training days of the place navigation, spatial probe test and visible platform trial, however the tendency was still to remain. Conclusion Purified water can damage the learning ability of offspring of rats and the learning ability delay will be more obviously when magnesium is deficient in food.