摘要
利用凝胶过滤层析Sephadex G-75、强阳离子交换色谱SP-650M,从苦瓜籽中分离纯化出一种活性多肽.该多肽在Tricine-SDS-PAGE上显示为单一的谱带,分子量约为6.8 kDa.在经还原剂二硫苏糖醇(DTT)处理后的电泳结果表明该多肽不含有分子间的二硫键.此多肽是一种胰蛋白酶抑制剂,同时具有抗植物致病菌活性,是一种具有双功能作用的多肽.
A novel antifungal peptide was isolated from bitter melon seeds by extraction with etch, Sephadex G75 chromatography and strong cation exchange chromatography column （SP -650M ）. It was identified to be homogeneous by Tricine - SDS - PAGE, and the relative molecular weight was estimated to be about 6.8 kDa. It was a trypsin inhibitor and showed antifungal activity against Pythium aphanidermatum, Fusarium oxysporum and Botrytis cinerea. It is expected to be applied to the prevention of insect pests and plant diseases.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期148-151,共4页
Journal of Fuzhou University(Natural Science Edition)
基金
福建省自然科学基金资助项目(C0310008)
关键词
苦瓜籽
纯化
胰蛋白酶抑制剂
抗真菌
bitter melon seeds
purification
trypsin inhibitor
antifungal activity
