离子色谱法(抑制电导)测定面粉中溴酸盐的含量

Determination of Bromate in Wheat Flour by Ion Chromatography with Suppressed Conductivity Detection

研究建立了离子色谱抑制电导测定面粉中溴酸盐的新方法。BrO3^-标准曲线的线性范围为0．05～5mg／L（r=0．9999），方法的加标回收率为81．7％～94．8％，相对标准偏差为2．3％，样品中BrO3^-的检出限为40μg／kg（以20g称样量计算）。实验表明，该方法快速、灵敏、准确，适合面粉中溴酸盐的测定。

The method developed in this work for the determination of bromate in wheat flour by ion chromatography with suppressed conductivity detector is described as follows： The separation of bromate is achieved on column with Metrosep A supp 5（250 mm × 4 mm i.d. ） and 3.2 mmol/L Na2CO3 ＋ 1.0 mmol/L NaHCO3 as eluent, and the detection is performed by conductivity detection. The linear range of the calibration curve of bromate is 0.05 - 5 mg/L with a correlation coefficient of 0.999 9. The recovery of spiked standard ranges from 81.7 % to 94.8 % and the relative standard deviation is 2.3 %. The determination limit of the method is 40 μg/kg of the bromate, calculated from a sample of 20 g. It is demonstrated that the proposed method is simple, sensitive and reliable for the determination of bromate in wheat flour.