摘要
目的:探讨宫颈癌组织放射治疗前后Aurora-A、p21基因的转录表达及其变化与临床放射治疗之间的关系。方法:运用RT-PCR的方法检测了103例宫颈癌组织中Aurora-A、p21的表达情况,放射治疗前后的变化。结果:103例宫颈癌组织中89例组织中(86.40%)检测至Aurora-A表达。经放疗后,63例(61.17%)表达减弱、7例(6.80%)表达增强,19例(18.45%)无明显变化,14例(13.59%)放疗前没有检测到Aurora-A表达的宫颈癌组织仍然没有检测到其表达。有75例(72.82%)组织表达p21。放射治疗后58例((56.31%)表达减弱,3例(2.91%)没有明显改变,14例(13.59%)表达增强。其余28例宫颈癌组织(27.18%)放疗前后均没有检测到p21表达。结论:放射治疗可以引起宫颈癌组织中Aurora-A和p21基因表达呈现降低趋势。因此,我们推测Aurora-A可能通过调节细胞周期及相关蛋白p21和c-myc等的功能,而与放射治疗效果存在一定联系。
OBJECTIVE： To investigate the expressions of Aurora - A and p21 genes in cervical cancer patients before and after receiving radiotherapy and the correlation between gene expression change and radiotherapy. METHODS： RT - PCR was applied to detect the expressions of Aurora A and p21 in cervical cancer tissues of 103 cases before and after radiotherapy. RESULTS： Of the 103 cases, 89 （86.40%） had expressions of Aurora - A. After radiotherapy, the expression of Aurora - A was down - regulated in 63 （61.17%）, up - regulated in 7 （6.80%）, and it was of no significant change in 19 （ 18.45 % ） ; however, there was still no expression of Aurora - A in the 14 cases （ 13.59 % ） whose expression of Aurora-A before radiotherapy was not detected. Of the total 103 cases, 75 （75.82%） had expression of P21. After radiotherapy, the expression of p21 was down - regulated in 58 （56.31% ）, up - regulated in 14 （ 13.59% ）, and it was of no significant change in 3 （2.91%）, however, there was still no expression of p21 in the 28 cases （27.18%） whose expression of p21 before radiotherapy was not detected. CONCLUSION： Down - regulation of the expressions of Aurora - A and p21 in cervical cancer patients after radiotherapy suggests that the correlation of Aurora - A with radiotherapeutic efficacy is possibly achieved through regulating the cellular cycle and the functions of p21 and c - myc etc.
出处
《中国医院用药评价与分析》
2008年第1期53-55,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
