摘要
为进一步探讨银耳多糖（ＴＰ）免疫调节作用的机制，建立了特异性荧光探针Ｆｕｒａ２测定脾细胞内游离钙离子浓度的方法，观察ＴＰ对脾细胞内游离钙离子浓度的影响。结果表明，ＴＰ在一定剂量范围内可以剂量依赖方式增加脾细胞内游离钙离子的浓度，并与ＣｏｎＡ有协同作用。在外钙为零时，ＴＰ对内钙释放无影响。
Effects of Tremella polysaccharides (TP) isolated from Tremella fuciformis Berk. on the cytoplasmic free calcium concentration in murine splenocytes were observed using Fura 2, a fluorescence indicator, technique. TP increased the cytoplasmic free calcium concentration of Fura 2 loaded murine splenocytes in a concentration dependent manner and showed synergetic effect with Con A. No effect was found in the release of intracellular calcium without extracellular calcium. Verapamil (10 μg·ml -1 ), a calcium channel blocker, was shown to inhibit the effect of TP on cytoplasmic free calcium, indicating that TP can affect the influx of extracellular calcium.
出处
《药学学报》
CAS
CSCD
北大核心
1997年第8期561-564,共4页
Acta Pharmaceutica Sinica
关键词
银耳多糖
钙
脾细胞
银耳
Tremella fuciformis Berk
Polysaccharides
Fura 2/AM
Cytoplasmic free calcium
