毛细管气相色谱法测定人血清中二十二碳六烯酸的浓度

DETERMINATION OF DOCOSAHEXENOIC ACID IN HUMAN SERUM BY CAPILLARY GAS CHROMATOGRAPH

毛细管气相色谱法测定人血清中二十二碳六烯酸的浓度李青翠张驰彭会明夏桂珠（北京医科大学药学院，北京１０００８３；山西省药品检验所，太原０３０００１）二十二碳六烯酸（ｃｉｓ４，７，１０，１３，１６，１９ｄｏｃｏｓａｈｅｘｅｎｏｉｃａｃｉｄ，Ｄ...

A reliable CGC method was developed for the determination of docosahexenoic acid (DHA) in human serum. The serum sample was acidified by adding two drops of 4 5 mol·L -1 H 2SO 4, and DHA was extracted from serum using ethyl acetate. The extract was evaporated to dryness under nitrogen stream. To each residue, 1 ml 1 3 mol·L -1 methanolic hydrogen chloride solution was added and the mixture was allowed to stand for 30 min in 60℃ water bath. After derivatization, the mixture was extracted with 1 ml of n hexane. The solvent was evaporated under a stream of nitrogen to dryness and the residue was dissolved in 30 μl n hexane and subjected to capillary GC, which was equipped with a fused silica capillary column (26 3 m×0 25 mm ID) coated with FFAP (free fatty acid phase, 0 1 μm film thickness). Tricosanoic acid was used as an internal standard. The retention time of DHA M and internal standard was 23 41 min and 20 79 min, respectively. The minimum detection concentration of DHA in serum was 40 ng·ml -1 with a serum volume of 200 μl and S/N value of 2. A good linear relationship between the peak area ratios and concentrations was found at the DHA concentrations ranging from 25 to 200 μg·ml -1 . The within day and between day precision was 5% and 9%, respectively.