庚型肝炎病毒(HGV/GBV-C)NS3 区基因序列的多变性

The sequence divergence in NS3 region of HGV/GBV C

用ＲＴ－ＰＣＲ方法从非甲、乙、丙、丁、戊型肝炎病人血清中检测庚型肝炎病毒。采用５６个循环周期的减温ＰＣＲ方法对ＮＳ３区基因进行扩增，并对阳性ＰＣＲ产物进行了克隆、测序。在ＮＳ３区，ＨＧＶ与样品之间核苷酸的同源性在８２．０％～９１．４％之间；而ＧＢＶ－Ｃ与样品之间的同源性为７８．４％～８４．２％之间。ＮＳ３区变异较大，这种变异可能与ＨＣＶ的基因变异的趋势相类似。

The new Hepatitis virus(HGV/GBV C) was detected in sera from nonA E hepatitis with using RT PCR.The reaction for amplifing NS3 region of HGV/GBV C was a single round touchdown PCR of 53 cycles with degenerate PCR primers.The positive products were cloned and sequenced.Nucleotide identity in NS3 between HGV and samples was between 82.0%￣91.4% and that between GBV C and samples was between 78.4%￣84.2%.So the sequence divergence of new hepatitis virus(HGV/GBV C) may be high.