靶向PEG10的siRNA真核表达载体对裸鼠肝癌移植瘤生长的影响

Influence of siRNA eukaryotic expression vectors targeting PEG10 gene on growth of transplanted human hepatocellular carcinoma in nude mice

目的探讨靶向PEG10的siRNA真核表达载体(psiRNA-PEG10)对裸鼠人肝癌移植瘤生长的影响。方法建立人肝癌细胞HepG2的荷瘤裸鼠模型,在接种后第14天分别将psiRNA空载体和psiR-NA-PEG10瘤内多点注射进行治疗,观察肿瘤大小变化、组织形态学及超微病理改变。结果psiRNA-PEG10治疗组肿瘤生长受到明显抑制,与生理盐水组和psiRNA组比较差异有显著性(P<0.001),抑瘤率明显高于其他两组,电镜下可见明显的细胞凋亡。结论靶向PEG10的siRNA真核表达载体对裸鼠肝癌移植瘤生长有抑制作用。

[Objective] To investigate the influence of siRNA eukaryotic expression vectors targeting PEG10 gene on growth of human transplanted human hepatocellular carcinoma in nude mice. [Methods] HepG2 cell line was implanted subcutaneously into nude mice. Fourteen days after implantation, psiRNA-PEG10 and psiRNA were injected into nude mice, respectively. The tumor volume was measured. The histopathological changes of the tumors were observed by HE staining and electron microscope. [Results] Growth of hepatoeellular carcinoma was significantly inhibited in the psiRNA-PEG10 therapy group as compared with that in the control group （P 〈0.001）. The growth inhibitory rate was remarkably higher than that of the two control groups, electron microscope revealed many apoptotic ceils. [Conduslon] Our finding suggest that psiRNA-PEG10 results in marked inhibition of hepatoeellular carcinoma growth in nude mice.