摘要
运用荧光光谱和紫外-可见吸收光谱法研究了锌酞菁与牛血红蛋白的相互作用。结果表明,锌酞菁与牛血红蛋白之间主要存在疏水作用,而且锌酞菁对牛血红蛋白还表现出较强的荧光猝灭作用。测定了不同温度下锌酞菁与牛血红蛋白相互作用的表观结合常数KA和热力学参数ΔG、ΔH、ΔS,基于能量转移机制计算了锌酞菁和牛血红蛋白结合时的作用距离r,并用同步荧光技术研究了锌酞菁对牛血红蛋白构象的影响。
The interaction between zinc phthalocyanine (ZnPc) and bovine hemoglobin (BHb) was investigated using UV/Vis and fluorescence spectroscopy. The experimental results showed that the fluorescence quenching of BHb by ZnPc was attributed to the formation of ZnPc-BHb complex; and static quenching was confirmed to result in the fluorescence quenching. The binding site numbern, the apparent binding constant KA and correspond-ing thermodynamic parameters were measured at different temperatures. The process of binding ZnPc molecule on BHb was a spontaneous molecular interaction procedure, in which the entropy increased and the Gibbs free energy decreased. The hydrophobic interactions played a major role in stabilizing the complex. The binding distancer and the energy-transfer efficiency E between ZnPc and BHb were obtained according to fluorescence resonance energy transfer; and the effect of ZnPc on the conformation of BHb was analyzed using the synchronous fluorescence spectroscopy.
出处
《化学试剂》
CAS
CSCD
北大核心
2008年第2期85-88,146,共5页
Chemical Reagents
基金
江苏省高校自然科学基础研究项目(07KJD150235)
江苏省“青蓝工程”资助项目
关键词
锌酞菁
牛血红蛋白
荧光猝灭
热力学参数
zinc phthaloeyanine
BHb
fluorescence quenching
thermodynamic parameters