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含survivin启动子重组的条件复制腺病毒联合顺铂对卵巢癌株HO-8910体外作用的实验研究

A novel conditionally replicating adenovirus combined with cisplatin(CDDP) enhanced the antitumorous effect on ovarian cancer cell line
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摘要 目的观察含survivin启动子的条件复制腺病毒CRAd-survivin-RGD4C联合顺铂对卵巢癌细胞株HO-8910的抑制作用。方法不同浓度的条件复制腺病毒CRAd-survivin-RGD4C病毒、顺铂以及该病毒联合顺铂体外处理人卵巢癌HO-8910细胞,四甲基偶氮唑蓝比色法检测人卵巢癌HO-8910细胞的生长情况,流式细胞技术检测细胞凋亡。结果该腺病毒对肿瘤细胞生长具有明显的抑制效应,并有浓度依赖性,联合化疗可以取得更佳的抑制肿瘤细胞生长的效果。结论溶瘤病毒联合顺铂治疗卵巢癌可能具有良好的前景。 Objective To investigate the effect of a novel conditionally replicating adenovirus CRAd - survivin - RGD in combination with cisplatin （CDDP） on ovarian cancer HO - 8910 cells. Methods Different doses of the virus CRAd, with or without the combination of CDDP, were used to infect the ovarian cancer HO -8910 cells. The cell viability was determined by MTT assay; the rate of apoptosis, by flow cytometry. Results The CRAd killed HO - 8910 cells in a dose - dependant manner. The inhibitory rate was higher in the combination treatment group than in the groups treated with CRAd alone or CDDP alone. Condusion CRAd virus combined with CDDP can be potentially a novel therapeutic agent for ovarian cancer.
作者 张蓓 刘嘉茵 韩素萍
机构地区 徐州市中心医院妇科 南京医科大学第一附属医院妇科
出处 《徐州医学院学报》 CAS 2008年第2期79-82,共4页 Acta Academiae Medicinae Xuzhou
关键词 卵巢癌 SURVIVIN 条件复制腺病毒 基因治疗 联合治疗 ovarian cancer survivin conditionally replicating adenovirus gene therapy combined therapy
分类号 R73-36 [医药卫生—肿瘤] Q782 [生物学—分子生物学]
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参考文献15

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二级参考文献2

  • 1鲁建国，第四军医大学学报，2000年，21卷，4期，464页
  • 2Rocco J W，Clin Cancer Res，1998年，4卷，7期，1697页

共引文献3

徐州医学院学报
2008年 第2期

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