响应面法优化八角茴香中莽草酸的超声波提取工艺研究

Optimization of the Ultrasound Assisted Extraction of Shikimic Acid from Illicium verum Hook. f. Using Response Surface Methology

采用响应面法优化超声波提取八角茴香中莽草酸的条件。在单因素试验的基础上,选取超声波作用时间、液料比和超声波功率为影响因子,应用Box-Behnken中心组合进行3因素3水平的实验设计,以莽草酸得率作为响应值,进行响应面分析(RSA)。结果表明,超声波法提取八角茴香中莽草酸的最佳提取条件为:1g八角茴香原料,以甲醇为溶剂,提取时间202s,液料比46.4∶1(mL∶g),超声波功率318W,得率预测值为8.25%,验证值为8.21%,与预测值的相对误差为0.45%。

Response surface analysis methodology （RSM） was used for optimizing the extraction process of shikimic acid from lllicium verum Hook. f., Based on single-factor experiments, three independent variables, namely：extracting time, solvent-solid ratio and ultrasound power, were selected as affecting factors during extraction. The experiments were arranged according to Box-Behnken central composite experiment design. Response surface analysis method was applied to determine the effect of central composite experiment design. The results showed that the optimum ultrasound extraction conditions for shikimic acid were as follow： 1 g material, extraction time 202 s, ultrasound power 318 W and ratio of liquid to solid 46.4：1 （mL： g）. The predicted shikimic acid extraction yield is 8.25 %, while the actual extraction yield is 8.21%, with relative error of 0.45 %.