竹笋水溶性多糖提取工艺研究

Study on Extraction Technology of Water-soluble Polysaccharides from Bamboo (Phyllostachys edulis (Carr.) Lehaie) Shoots

采用单因素试验和正交试验设计,探索了毛竹笋水溶性多糖提取的最佳工艺条件。结果表明,影响竹笋水溶性多糖得率的因素主次关系依次是乙醇体积分数>提取温度>提取时间>水料比;多糖的最佳提取工艺条件为:水料比10∶1(mL∶g),提取时间4h,提取温度100℃,95%乙醇醇析。在此最佳工艺条件下,竹笋水溶性多糖的得率为0.32%。

In order to study the optimum technological conditions for extracting water-soluble polysaccharides from bamboo （Phyl- lostachys edulis（Carr. ） Lehaie） shoots, the effects of each factor such as ratio of water to sample, extraction time, extraction temperature and ethanol volume fraction on the extraction of the water-soluble polysaccharides of bamboo shoots were investigated, using the methods of single factor test and orthogonal design test of L9 （34 ）. The results showed that the main factors affecting the yield of polysaccharides were sequenced as follows ： ethanol volume fraction 〉 extraction temperature 〉 extraction time 〉 ratio of water to sample; the optimum technological conditions were as follow： ratio of water to sample 10 ： 1 （ mL ： g）, extraction time 4 h, extraction temperature 100 ℃ and the ethanol volume fraction 95 %. Using the optimum technological conditions, the yield of the water-soluble polysaccharides of bamboo shoots was 0.32 %.