脑缺血再灌流大鼠海马hsp70及bcl-2基因的表达

THE EXPRESSION OF hsp70 AND BCL 2 GENES IN HIPPOCAMPUS OF THE RATS EXPOSED TO CEREBRAL ISCHEMIA AND REPERFUSION

为进一步了解中枢神经系统中选择性易损伤的分子机制，采用大鼠短暂前脑缺血再灌流损伤动物模型，应用Ｎｏｒｔｈｅｒｎ杂交、原位杂交及免疫组织化学方法，检测了ｈｓｐ７０及ｂｃｌ－２基因的表达及其组织学分布。发现易损伤的海马ＣＡ１区锥体细胞出现ｈｓｐ７０基因的诱导表达，ＢＣＬ－２蛋白合成受抑制；而耐受缺血的海马ＣＡ３区锥体细胞则明显地持续表达ＢＣＬ－２蛋白，却未见明显的ｈｓｐ７０基因表达。因此提示，ｈｓｐ７０基因的表达是神经元缺血的应激指征，也可能对神经元有保护作用；

In order to study the molecular mechanism of the selective vulnerability in central nervous system,the expression and distribution of hsp70 and BCL 2 gene were detected by using Northern blot analysis , in situ hybridization and histochemistry method in transient forebrain ischemia and reperfusion rats It was found that hsp70 gene expression occurred and the synthesis of BCL 2 protein was inhibited in hippocampalCA 1 region vulnerable to ischemia,while BCL 2 protein was stained strongly and the signals of hsp70 were not observed in CA 3 region resistant to ischemia The results indicate that the expression of hsp70 gene may be not only as a marker for neuron ischemia,but also play a protective role in the neuronal injury BCL 2, meanwhile, may have neuro protective effect on the ischemic neurons