摘要
本工作采用无血清原代培养大鼠肝细胞法，观察了重组人肝细胞生长因子（ｒ－ｈＨＧＦ）对四氯化碳（ＣＣｌ４）致大鼠肝细胞损伤的保护作用。结果表明，ｒ－ｈＨＧＦ对ＣＣｌ４染毒肝细胞有明显的保护作用。ｒ－ｈＨＧＦ保护组较ＣＣｌ４染毒组细胞存活率显著升高，细胞内丙氨酸转氨酶、钾离子漏出明显降低。结果提示，ｒ－ｈＨＧＦ可减轻ＣＣｌ４对肝细胞膜的损伤。
The protective effect of recombinant human hepatocyte growth factor (r hHGF) against carbon tetrachloride (CCl 4) injury in cultured rat hepatocytes was investigated. It was found that r hHGF had cytoprotective effect on hepatocytes anainst CCl 4 poisoning. Pretreatment with r hHGF significantly increased the viability of cultured hepatocytes, reduced the leakage of intracellular alanine transaminase and potassium into the culture medium, as compared with the CCl 4 group. The results suggest that r hHGF may protect hepatocytes against CCl 4 injury through lessening the severity of membrane lesion,preventing intracellular alanine transaminase and potassium leakage and sustaining the integrity of hepatocyte membrane.
出处
《中国应用生理学杂志》
CAS
CSCD
1997年第3期228-230,共3页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
关键词
肝细胞生长因子
四氧化碳
肝细胞
原代培养
损伤
recombinant human hepatocyte growth factor (r hHGF)
alanine transaminase (ALT)
potassiumions
carbon tetrachloride (CCl 4)
primary hepatocyte culture
