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促甲状腺激素受体第十外显子基因突变与甲状腺癌发病的相关性 被引量：2

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摘要 目的研究甲状腺癌发病是否与促甲状腺激素受体(TSHR)第十外显子基因突变有关。方法采用巢氏-多聚酶联反应-单链构象多态性分析方法(NEST-PCR-SSCP)及DNA测序方法,对76例甲状腺癌(其中10例滤泡型癌,65例乳头状癌,1例髓样癌)和44例对照组织TSHR第十外显子基因进行检测。结果经NEST-PCR-SSCP检测甲状腺癌TSHR第十外显子未发现明显带型异常,第三片段(即第3胞内环)取2例对照组织和5例甲状腺癌组织进行DNA测序,7例组织TSHR2000位点碱基均由C→T,使得所编码的601位氨基酸由组氨酸(CAT)→酪氨酸(TAT)(His→Tyr),余无明显基因突变。结论甲状腺癌TSHR第十外显子未发现基因突变,提示甲状腺癌发病与TSHR第十外显子基因突变无关;由于所测7例标本601位氨基酸均为酪氨酸,考虑中国人TSHR基因与国外人群存在差异。
作者 戴亚丽 张帆 姜志茹 叶静 龙霞 蓝薇 彭卫群
机构地区 北京大学深圳医院内分泌科 北京大学深圳医院老年病科 北京大学深圳医院中心实验室
出处 《广东医学》 CAS CSCD 北大核心 2008年第3期382-384,共3页 Guangdong Medical Journal
基金 广东省深圳市科技局资助项目(编号:200104014)
关键词 甲状腺癌 促甲状腺激素受体 第十外显子 单链构象多态性分析 基因多态性
分类号 R771.3 [医药卫生—眼科] R736.1 [医药卫生—肿瘤]
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参考文献9

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同被引文献37

  • 1郑冬梅,鹿宁,陈青,张海清,姜秀云,管庆波,赵家军.甲状腺结节的临床分析[J].山东大学学报（医学版）,2009,47(8):14-17. 被引量：31
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广东医学
2008年 第3期

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