摘要
采用响应曲面法对玉米秸秆酶水解反应进行了评价,从而确定玉米秸秆酶水解反应的优化条件为底物中酶浓度57.5 FPU/g,底物质量浓度64.7 g/L,温度48℃,pH值4.8,反应时间49 h。在此条件下,每100 g底物还原糖产量为46.34 g,与模型预测值46.43 g底物非常接近。与单因子实验相比,酶浓度降低了2.5 FPU/g,底物质量浓度提高了14.7 g/L,反应时间缩短了23 h,还原糖产量提高10.41%。响应曲面法优化玉米秸秆酶水解是合理有效的,得到的优化条件有利于玉米秸秆的转化生产还原糖。
Response surface methodology was adopted to evaluate the enzymatic hydrolysis of corn stover. Optimal levels for each variable under the highest reducing sugar yield were obtained, including enzyme concentration 57.5 FPU/g in substrate, substrate mass concentration 64.7 g/L, temperature 48 ℃, pH 4.8 and reaction time 49 h. And a reduced sugar yield of 46.34 g （per 100 g substrate） was achieved, which was consistent with the predicted value 46.43 g （per 100 g substrate）. Compared with one-factor-at-time experiments, enzyme concentration decreased by 2.5 FPU/g, substrate mass concentration increased by 14.7 g/L and reaction time dropped to 23 h, and reduced sugar yield increased by 10.41%. surface methodology is reasonable and efficient; to sugar. Optimization of enzymatic hydrolysis corn stover using response the optimal conditions are advantageous for converting corn stover
出处
《化学工程》
EI
CAS
CSCD
北大核心
2008年第2期59-62,共4页
Chemical Engineering(China)
关键词
玉米秸秆
酶水解
还原糖
响应曲面法
corn stover
enzymatic hydrolysis
reduced sugar
response surface methodology
