国产硫酸头孢喹肟对小鼠的急性和蓄积毒性研究

Study on Acute Toxicity and Cumulative Toxicity of Domestic Cefquinome Sulfate in Mice

为了研究国产硫酸头孢喹肟对昆明小鼠的急性毒性和蓄积毒性,采用改良寇氏法测定国产硫酸头孢喹肟的LD50;以剂量定期递增法计算蓄积系数,并于给药后第21天给予试验组和对照组小鼠1 LD50的量,观察各组小鼠对硫酸头孢喹肟的耐受性。结果表明,国产硫酸头孢喹肟原料药对小鼠口服LD50大于5 000 mg/kg,其混悬液制剂对小鼠腹腔注射LD50为844.03 mg/kg,LD50的95%可信限为802.05 mg/kg^887.56 mg/kg;蓄积试验中无小鼠死亡,蓄积系数K>5.3;耐受性试验显示,试验组死亡率(10%)显著低于对照组(40%)(P<0.05)。由此可见,国产硫酸头孢喹肟毒性低,临床使用安全,对小鼠没有蓄积毒性,小鼠对本品可产生明显耐受性。

Study on acute toxicity and cumulative toxicity of domestic cefquinome sulfate in mice, The LD50 of cefquinome sulfate was detected by improved Karber method. The accumulative coefficient was detected by accumulative coefficient method. After administration, at the 21st day, the experimental group and control group were administered with 1LD50 dose. The tolerance of cefquinome sulfate in each group mice was observed. The mouse oral LD50 of cefquinome sulfate was higher than 5 000 mg/kg, LD50 arrived at 844.03 mg/kg after intraperitoneal injection of its suspension and 95 % confidence interval was 802.05 mg/ kg to 887.56 mg/kg. The accumulative coefficient K was higher than 5.3, and no death occurred. The mortality of mice in test group was significantly lower than that in control group. The toxicity of domestic cefquinome sulfate is low,and the drug is safe and can be used in clinic. There is no cumulative toxicity in mice; The mice showed the obvious tolerance to this drug.