RT-PCR技术检测呼吸道合胞病毒RNA方法的建立

Establishment of Method for Detection of Respiratory syncytical Virus RNA Using Reverse-transcription Polymerase Chain Reaction

为了建立一种快速诊断呼吸道合胞病毒（RSV）感染的方法，根据RSVN基因的核苷酸序列，设计合成了一对引物，经RT-PCR扩增，可检出RSVRNA该引物不能检测流感病毒、副流感病毒RNA。应用该法可检出疑为RSV感染的婴幼儿鼻咽分泌物中的病毒RNA且比病毒分离法敏感，特异性与免疫荧光法一致。结果表明RT－PCR法具有快速、敏感、特异的优点，可用于RSV感染患儿的临床诊断。

A method of reverse - transcription polymerase chain reaction(RT - PCR) was established for detection of respiratory syncytical virus (RSV) RNA genome, The primers were designed based on the nucleotide sequence of RSV N gene. The results showed that the primers can't amplify the genomes of influenza virus and parainfluenza virus, but it can detect RSV genome in naSopharyngeal aspirate samples in infants and young children. This method is better than virus isolation. Its specificity is similar to immunofluorescence assay. The results indicated that the RTPCR for RSV RNA is specific, rapid and sensitive. It is suitable for clinical diagnosis of RSV infection.