HHV-6体外感染对外周血单个核细胞IL-6、IL-8的诱生NK活性的影响

Effect of HHV-6 Infection on Cytokines IL - 6,IL-8 Induction and NK Activity of Human Peripherial Blood Mononuclear Cells

采用生物活性法和／或酶联免疫吸附法以及乳酸脱氢酶释放法，研究了人类疱疹病毒6型（HHV－6）南京地方株CNS对人外周血单个核细胞（PBMCs）的IL—6、IL－8的诱生和NK活性的影响，并与国外的GS株作比较。结果发现，HHV—6CN8、GS两株病毒感染均可诱导PBMCs产生IL－8，48h达到峰值。两株病毒所诱生的IL－8水平并无显著性差异（P＞0．05），并可抑制IL－6的产生，但GS株的抑制作用强于CNS株（P＜0．05）。HHV－6体外感染12～24h可以增强NK活性，且CN8株诱导的NK活性高于GS株（P＜0．05），之后NK活性逐渐减弱。以上结果提示：HHV－6感染可以通过诱生细胞因子和改变NK活性而影响人的免疫功能，而A组的GS株对免疫功能的抑制作用大于B组的CN8株。

The effects of HHV - 6 infection in vitro on cytokines such as IL - 6, IL - 8 synthesis and NK activity of human peripherial blood mononuclear cels (PBMC) were studied by comparing CN8 strain with GS strain. Interleukin - 8 (IL - 8) could be detected as eary as 24 h, plateaued at 48h or so by ELISA. There were significant differences of IL - 8 leve between the control groop and HHV -6 GS or CN8 strain infection groups- Comaring with uninfected cells, synthesis of IL - 6 in infected cells was inhibited slightly determined by biological activity assay and ELISA. The inhibition activity of GS strain was significantly higher than that of CN8 strain, By LDH releasing assay, activity of NK was augumnted 24 h after infection, then graduanlly decreased. The NK activity stimulated by GS strain was lower than that of CN8 strain- All these results indicated that HHV - 6 could disturb function of human immunoloical Cells and immunity inhibitbo activity of GS strain (A variant) was more remarkable than tha of CN8 strain (B varian).