甲型肝炎病毒细胞受体结合区氨基酸突变分析

Assay of Amino Acid Mutagenesis in Hepatitis A Virus Cellular Receptor Binding Region

利用PCR引导的基因突变技术，对位于甲型肝炎病毒农壳蛋白VP1上的细胞受体结合区进行氨基酸定点突变。结果发现当第1143、1187、1202和1225位氨基酸发生突变时，突变株病毒在细胞中的增殖动力学改变，病毒增殖量呈不同程度减少，提示这些位置上的氨基酸可能与细胞受体结合。若发生突变将影响病毒对敏感细胞的吸附和脱衣壳过程，使病毒感染力下降。

Point mutation was produced in the cellular receptor binding region of hepatitis A virus VP1 based on PCR directed mutagenesis. The results showed that the replication kinetics were changed when 1143, 1187, 1202 and 1225 amino acid were mutated respectively. HAV replicatioll quantity decreased at a different range compared with prototype HAV strain. The results indicated that these amino acid may be involved in the binding of cellular receptor, The amino acid substitution may affect the attachment and the uncoating process of HAV to permissive cell,therefore the virus infection decreased.