广东烟草花叶病毒株系研究

Studies on Strains of TMV in Tobacco Production Areas in Guangdong Province

烟草花叶病毒（TMV）引起的烟草花叶病在广东不同的烟草主产区存在着症状的差异。从广东梅州和南雄烟区采集的138个TMV标样中，选取6个不同症状的分离物作为研究对象，发现它们在不同种属寄主和不同烟草品种上表现明显的TMV普通株特征症状，虽然在某些寄主上其症状也有一些差异，但未能反映株系间的差异。这6个分离物具有几乎相同的病毒粒体形态、电泳迁移率、钝化温度以及紫外吸收特征。经琼脂双扩散法和间接ELISA法的血清学反应，表明它们具较强的血清学关系。因而它们有很强的同源性。经病毒粒体外壳蛋白的氨基酸分析，在氨基酸总数上，分离物GD1和GD3各有156个氨基酸．GD2和GD4各有159个，GD5和GD6分别有158和157个氨基酸。上述分析试验结果初步表明，在广东不同烟草主产区引起的症状有所差异的6个分离物均属IMV普通株。

Six Tobacco - TMV isolates which cause different symptom expressions on tobacco in different tobcco production areas in Guangdog Province were selected from 138 samples and were studied. The symptom expression of the 6 Tobacco - TMV isoates on 6 differenial host plants and 6 tobacco varieties were about the same, although some minor differences might exist. The morphology of the virus particles of the 6 isoates, their thermal inactivation points, electrophoretic mobility and ultraviolet light absorption were found to be about the same. By agar donble - diffusion reaction and indirect ELISA, the 6 isolates were found to have very close serological relationship. The amino acid compositions of the coat proteins of the 6 isolates were found to be very siwhar each other and similar to the Vulgare strain, althonh minor dihaences ekisted in the amount of certain amino residues. According to the experimental results, it was conluded that the 6 isoates all belong to the common strain of TMV.