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三种夜蛾科昆虫核型多角体病毒DNA相关性的研究

Studies on Relationship of Three Nuclear Polyhedrosis Virus Genomes from Noctuid Insects
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摘要 MbNPV ,SeNPV and SINPV are important biological agents for three noctuid insects Mamestra brassicae, Spodoptera exigua and Spodoptera litura, respectively. The genomic DNAs from these NPVs were analysed by restriction and dot hybridization. The results showed that they have different restriction maps and low homology among MbNPV, SeNPV and SlNPV. The polyhedrin gene of MbNPV was localized under non - stringency by using 32P - labelled Buzura suppressaria NPV polyhedrin gene as a probe. MbNPV ,SeNPV and SINPV are important biological agents for three noctuid insects Mamestra brassicae, Spodoptera exigua and Spodoptera litura, respectively. The genomic DNAs from these NPVs were analysed by restriction and dot hybridization. The results showed that they have different restriction maps and low homology among MbNPV, SeNPV and SlNPV. The polyhedrin gene of MbNPV was localized under non - stringency by using 32P - labelled Buzura suppressaria NPV polyhedrin gene as a probe.
出处 《中国病毒学》 CSCD 1997年第3期278-280,共3页 Virologica Sinica
基金 中国科学院生物分类区系专项资助
关键词 核型多角体病毒 DNA 相关性 Nuclear polyhedrosis virus(NPV), Restriction enzymes analysis, Dot hybridization,Polyhedrin gene
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