沈阳市副溶血弧菌重复序列PCR分型

Typing of vibrio parahaemolyticus clinical isolated strains from Shenyang region by repetitive element sequence-based PCR

目的探讨重复序列PCR对临床分离株副溶血弧菌进行分型的可行性;从分子水平了解沈阳市流行的副溶血弧菌菌型特征。方法利用基因外重复回文序列-PCR(PEP-PCR)和肠细菌基因间共有重复序列-PCR(ERIC-PCR)对40株临床分离副溶血弧菌基因组DNA进行扩增,对扩增的DNA电泳指纹图谱进行分型分析。结果40株副溶血弧菌呈现不同程度的基因多态性。基因外重复回文序列-PCR分辨力指数可达到0.953,40株菌株分为16个型,优势菌型为G型,占总菌数的20.0%。肠细菌基因间共有重复序列-PCR分辨力指数为0.5;40株菌株可分为4个型,优势菌型为D型,占总菌数的67.5%。结论重复序列PCR可以用于副溶血弧菌临床分离株的分子分型研究,其分型敏感程度优于血型分型。

Objective To understand the epidemic and distribution characteristic of vibrio parahaemolyticus in Shenyang,analyze the typing possibility of vibrio parahaemolyticus by repetitive element sequence-based PCR.Methods Repetitive Extragenic Palindromic PCR and Entero-bacterial Repetitive Intergenic Consensus PCR were used to generate DNA fingerprints for vibrio parahaemolyticus.Results 40 clinical strains of vibrio parahaemolyticus,representing a wide range of DNA patterns,were grouped into 16 and 4 patterns,with discrimination indexes of 0.953 and 0.5 by REP-PCR and ERIC-PCR,respectively.Pattern G was predominant,accounting for 20.0% of total count,according to REP fingerprints,and pattern D was predominant,67.5% of total count,according to ERIC fingerprints.Conclusion Repetitive element sequence-based PCR can be used to type vibrio parahaemolyticus,which is super to the serotyping method.