香蕉花叶病检测技术的比较

DETECTION TECHNIQUES OF BANANA MOSAIC DISEASE

香蕉花叶病检测技术的比较＊李华平胡晋生＊＊范怀忠（华南农业大学植保系，广州５１０６４２）关键词香蕉，黄瓜花叶病毒（ＣＭＶ），ＥＬＩＳＡ，核酸斑点杂交（Ｄｏｔ－ｂｌｏｔ），反转录－聚合酶链反应（ＲＴ－ＰＣＲ）香蕉花叶病已成为阻碍华南香蕉产区香蕉生产的重...

ELISA,Dot-blot and RT-PCR were developed and evaluated for detection of the virus in diseased banana plants.RT-PCR was the most sensitive assay when compared with Dot-blot and ELISA.The Dot-blot assay was 100 times more sensitive than ELISA.The distribution of CMV within banana plants was uneven,CMV concentritions were higher in younger leaves than in older ones,as well as higher in the midribs than the leaves.The CMV-Oahu isolate was generally not detected in banana by ELISA using antibodies to both CMV subgroups Ⅰ and Ⅱ,but was positive in dot-blot and RT-PCR tests.