双向凝胶电泳分析糖尿病大鼠肝脏蛋白质表达

Protein expression in the Liver of diabetic rats by two-dimensional gel electrophoresis

目的用双向凝胶电泳分析糖尿病大鼠肝脏蛋白质表达，探讨糖尿病引起肝脏生物化学改变的机制。方法采用链脲佐菌素（STZ）诱发Wistar大鼠产生高血糖，建立1型糖尿病大鼠模型。用固相pH梯度双向凝胶电泳（2D-PAGE）分离糖尿病大鼠与正常肝脏的蛋白质表达图谱。考马斯亮蓝染色凝胶后，用Image Master 2D Platinum软件进行图像分析。结果图像分析测得2块胶的匹配率迭66％，在等电点pH3～10、分子量14．0～75．4kD范围内分离得糖尿病大鼠肝脏的蛋白点大约950个，正常大鼠的蛋白点大约1069个，其中至少51个蛋白点在两种肝脏间有2倍以上量变。结论糖尿病引起肝脏蛋白质表达发生变化，糖尿病大鼠肝脏蛋白质表达的研究有助于探讨糖尿病并发症的发生机制。

Objective To examin the difference in the protein profiling of liver between the diabetic rats, and to study the changed course of liver under Diabetes Mellitus （DM）. Methods Using STZ stimulated Wistar rats induced high level of blood glucose, then the TIDM of rots model was made successfully. The protein of liver of DM rats and normal rats were analyzed by two-dimensional gel electrophresis and imaging analysis. Results The ratio of matched protein spots between two gels was up to 66%. 950 and 1 069 protein spots with a molecular mass between 14. 4-97. 4 kD, and isoelectrie points （pI） from 3-10 were catalogued from co-stained gels of the liver. At least 51 proteins were upregulated or down-regulated by over two folds between two groups. Conclusion The different expression of protein profile may be a underlying course of Diabetic liver.