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线粒体K_(ATP)通道开放剂对培养大鼠心肌细胞缺血/再灌注损伤心肌线粒体的保护作用 被引量：2

Mitochondrial ATP-sensitive potassium channel opener protectes mitochondrial functions from simulated ischemia/reperfusion injury in cultured cardiac myocytes
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摘要 目的探讨线粒体ATP敏感性钾通道开放剂二氮嗪预处理对大鼠心肌细胞线粒体功能保护作用。方法采用培养大鼠心肌细胞缺血/再灌注损伤模型,观察二氮嗪预处理对心肌细胞缺血/再灌注损伤损伤后细胞线粒体活性和线粒体膜电位影响。结果二氮嗪预处理大鼠后,对培养大鼠心肌细胞缺血/再灌注损伤有保护作用,能减少心肌细胞线粒体活性和线粒体膜电位的下降。结论线粒体ATP敏感性钾通道开放剂能产生预处理心肌细胞线粒体保护效应。 Objective To investigate the protection of preconditioning by mitochondrial ATP-sensitive potassium channel opener from simulated ischemia/reperfusion injury in cultured cardiac myocytes. Methods In model of simulated ischemia/reperfusion injury,rats were pretreated with diazoxide. The activity of mitochondria, mitochondrial membrane potential were observed. Results Compared with the controls the activity of mitochondria, mitochondrial membrane potentia were decreased in ischemia/reperfusion group. Administration of diazoxide increased the activity of mitochondria and mitochondrial membrane potential. Conclusion Preconditioning by diazoxide can protect the function of myocardial mitochondria against myocardial ischemia/reperfusion injury.
作者 黄河 肖颖彬 杨天德 李洪 李永旺 陈建
机构地区 第三军医大学新桥医院麻醉科 第三军医大学新桥医院心血管外科 第三军医大学高原军事医学系
出处 《重庆医学》 CAS CSCD 2008年第5期479-480,483,共3页 Chongqing medicine
基金 国家自然科学基金资助项目(No.30500211)
关键词 线粒体ATP敏感性钾通道 缺血/再灌注 膜电位 mitochondrial ATP-sensitive potassium channel ischemia/reperfusion membrane potential
分类号 R363 [医药卫生—病理学]
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参考文献9

  • 1李洪,肖颖彬,杨天德.二氮嗪预处理对培养成年大鼠心肌细胞模拟缺血/再灌注损伤的保护作用及其与剂量的关系[J].重庆医学,2004,33(11):1611-1613. 被引量：6
  • 2刘荣国,汪炜健,宋娜,陈彦青,李立环.二氮嗪预处理上调Bcl-2/Bax蛋白比值减少缺氧复氧所致体外培养的海马神经元凋亡(英文)[J].生理学报,2006,58(4):345-350. 被引量：12
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二级参考文献26

  • 1李洪,肖颖彬.成年大鼠心室肌细胞的分离、培养与鉴定[J].第三军医大学学报,2004,26(7):644-646. 被引量：18
  • 2Garlid KD, Paucek P, Yarov-Yarovoy V, et al.Cardioproctective effect of diazoxide and its interaction with mitochondrial ATP-sensitive K+channels:possible mechanism of cardioprotection[J]. Circ Res, 1997, 81: 1072
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  • 4Tsouka V, Markou T, Lazou A. Differential effect of ischemic and pharmacological preconditioning on PKC isoform translocation in adult rat cardiac myocytes[J]. Cell Physiol Biochem, 2002,12(5-6): 315
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  • 9Wakahara N, Katoh H, Yaguchi Y, et al.Difference in the cardioprotective mechanisms between ischemic preconditioning and pharmacological preconditioning by diazoxide in rat hearts[J]. Circ J, 2004,68(2):156
  • 10Hanley PJ, Mickel M, Lffler M, et al. K(ATP) channel-independent targets of diazoxide and 5-hydroxydecanoate in the heart[J]. J Physiol, 2002,542:735

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重庆医学
2008年 第5期

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