心外膜应用腺苷时c-fos在脊髓、延髓和丘脑中的表达

EXPRESSION OF c-fos IN SPINAL CORD,MEDULLA OBLONGATA AND THALAMUS FOLLOWING EPICARDIAL APPLICATION OF ADENOSINE

在12只切断两侧缓冲神经和迷走神经的麻醉大鼠，观察了心外膜应用腺苷对脊髓、延髓和丘脑c－fos原癌基因表达的影响。结果显示：在心外膜应用腺着组大鼠，动脉血压和心率无明显变化；脊髓T3节段背角、延髓巨细胞旁外侧核（PGL）以及丘脑的腹后外侧核（VPL）、后核（Po）、中央外侧核（CL）和束旁核（PF）等部位Fos蛋白样免疫阳性反应（FLI）神经元显著增加；而在溶剂对照组大鼠，仅见少数FLI细胞。以上结果提示，心外膜应用眼苷可引起脊髓、延髓和丘脑等多个部位与痛觉有关的神经元兴奋。

Effects of epicardial application of adenosine on the expression of c-fos proto-oncogene in spinal cord, medulla oblongata and thalamus were examined in 12 sinoaorticdenervated and vagotomized anesthetized rats. The results showed that following epicardial application of adenosine the Fos-like protein immunoreactive (FLI) neurons wereremarkably increased in the dorsal horn of T3 spinal segment, nucleus paragigantocellularis lateralis (PGL) of medulla oblongata, ventral postero-lateral thalamic nueleus(VPL), posterior thalamic nucleus (Po), parafascicular thalamic nucleus (PF) and centrolateral thalamic nucleus (CL), while the blood pressure and heart rate were not affected. Only a few FLI neurons were found in the vehicle-control rats. The results indicate that epicardial application of adenosine may activate the pain-related neurons inspinal cord, medulla oblongata and thalamus.