温室黄瓜叶部微生物区系

Phyllosphere and endophytic microflora in greenhouse cucumber leaves

采用稀释分离法和消毒叶片研磨液培养法对温室黄瓜叶围和内生微生物进行了分离,共分离到248个菌株,初步鉴定出13个属的叶围真菌,其中链格孢属(Alternaria)和青霉属(Penicillium)真菌为优势类群;鉴定出4个属的内生真菌,其中曲霉属(Aspergillus)真菌为优势类群;10个属的叶围细菌,其中芽孢杆菌属(Bacillus)和黄单胞菌属(Xan-thomonas)细菌为优势类群;6个属的内生细菌,其中芽孢杆菌属和假单胞菌属(Pseudo-monas)细菌为优势类群;6个属的叶围酵母菌,其中隐球酵母属(Cryptococcus)为优势类群;已鉴定出2个属的叶围放线菌,分别为链霉菌属(Streptomyces)和小多孢菌属(Micropolpspora)。未分离到内生酵母菌和放线菌。

The phyllosphere and endophytic microflora in greenhouse cucumber leaves at different growth stage were analyzed by diluting isolation with selective media. A total of 248 microbial strains were isolated, and 13 genera of phyllosphere fungi with Alternaria and Penicillium as the dominant, 4 genera of endophytic fungi with Aspergillus as the dominant, 10 genera of phyllosphere bacteria with Bacillus and Xanthomonas as the dominant, 6 genera of endophytic bacteria with Bacillus and Pseudomonas as the dominant, 6 genera of phyllosphere yeasts with Cryptococcu as the dominant, and 2 genera of phyllosphere actinomycetes （ Streptomyces and Micropolpspora） were identified. No endophytic yeasts and actinomycetes were isolated.