摘要
本实验在培养的乳鼠心肌细胞缺氧复氧（A／R）损伤模型上观察碱性成纤维细胞生长因子（bFGF）对A／R损伤及蛋白激酶C（PKC）活性的影响，以探讨bFGF作为药物预处理心肌保护的可行性及其机理。结果表明，bFGF预处理呈浓度依赖性地提高A／R后心肌细胞存活率，减少细胞内ATP消耗及胞浆乳酸脱氢酶（LDH）漏出；PKC抑制剂H7完全消除bFGF的上述保护作用；实验结果还表明，bFGF可以直接激活心肌细胞PKC，其激活时相的变化与缺氧预处理者相近，提示bFGF对心肌细胞A／R损伤的保护作用可能是由PKC所介导的。
The effects of basic fibroblast growth factor (bFGF) on anoxia/reoxygenation(A/R) injury and protein kinase C (PKC) activity were studied on a model of A/R injury of neonatal rat cardiomyocytes to investigate the possibility of its using as a substrate for pharmacological preconditioning. The data indicated that bFGF improved theviability of cardiomyocytes, lowered the deplection of ATP and leakage of intracellularlactate dehydrogenase (LDH) in a concentration-dependent manner. PKC inhibitor, H7,completely abolished the protective effects. It was also found that bFGF directely activated PKC in cardiomyocytes in a time course similar to that in hypoxic preconditioning.The data suggested that the protective effect of bFGF on cardiomyocyte A/R injurymight be mediated by PKC.
出处
《生理学报》
CAS
CSCD
北大核心
1997年第4期455-458,共4页
Acta Physiologica Sinica
基金
国家自然科学基金!39570300
关键词
心肌细胞
缺氧复氧损伤
成纤维细胞
生长因子
basic fibroblast growth factor
cardiomyocyte
preconditioning
protein kinase C
anoxia/reoxygenation injury
