大鼠下丘脑离体脑薄片视上核神经元的全细胞记录

WHOLE-CELL RECORDINGS OF THE SUPRAOPTIC NUCLEUS NEURONS FROM RAT HYPOTHALAMIC SLICES IN VITRO

在大鼠下丘脑薄片标本上对52例视上核神经元进行了全细胞膜片箝记录。膜被动及主动电生理参数测量如下：静息电位，59±8mV；输入阻抗，535±129MΩ；时间常数，32±9ms；动作电位幅度，99±11mV；超射值，37±13mV（n＝39）。大多数神经元在接受去极化刺激时出现明显的慢后超极化电位或电流。我们发现，在电压箱状态下几乎所有的视上核神经元均接受兴奋性和／或抑制性突触传λ（n=13）。药理学实验表明，兴奋性突触后电流是由non－NMDA亚型谷氨酸受体介导，而抑制性突触后电流由GABAA受体介导。

Fifty-two supraoptic nucleus neurons in rat slice preparations were studied usingwhole cell patch-clamp technique- The mean passive and active membrane propertieswere measured as follows: resting membrane potential, 59± 8 mV; input resistance,535±129 MΩ; time constant, 32± 9 ms; amplitude of the action potentials, 99±11mv; overshoot, 37±13 mV (n= 39 ). Most of these neurons showed a prominent slowafter-hyperpolarization potential or current in response to depolarizing pulse. In votageclamp condition, it was found that virtually all supraoptic neurons (n=13) were invaded by spontaneous synaptic inputs. Pharmacological experiments showed that the excitatory postsynaptic currents (EPSCs) were mediated by non-NMDA glutamate receptors,whereas inhibitory postsynaptic currents (IPSCs) by GABAA receptors.