从绵羊脂肪组织提取总RNA方法的改进及应用

Improvement of methods for extracting high quality total RNA from sheep adipose tissue and its usage

以绵羊脂肪组织为材料,对脂肪组织总RNA提取方法进行了改进,并分析了脂肪酸合成酶基因(fattyacid synthase,FAS)在绵羊腹部皮下脂肪组织、肠系膜脂肪组织和尾部脂肪组织中表达的差异性.结果表明:利用改进后的方法从绵羊脂肪组织中提取出的RNA无DNA污染、均一、完整、质量可靠.绵羊腹部皮下脂肪中FASmRNA的表达量极显著高于尾部脂肪组织和肠系膜脂肪组织(P<0.01),且尾部脂肪组织和肠系膜脂肪组织中FAS mRNA的表达量差异不显著(P>0.05).

An improved method of extracting the total RNA from ventral subcutaneous adipose tissue, mesentery adipose tissue and tailhead adipose tissue of sheep was developed and the expression and tissue distribution of the fatty acid synthase（FAS）gene in sheep were investigated. The result showed that：total RNA extracted from adipose tissue by the improved method was pure, integral, high quality and without chromosome DNA contamination. The expression of FAS mRNA in the ventral subcutaneous adipose tissue were significantly different compared with tailhead adipose tissue and mesentery adipose tissue （P〈0.01）,but there was no significant difference between tailhead adipose tissue and mesentery adipose tissue（P〉0. 05）.