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RLFmRNA在妊娠大鼠卵巢中的表达 被引量：1

EXPRESSION OF RLFMRNA IN RAR OVARY DURING PREGNANCY
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摘要 目的:观察松弛素样因子(RLF)基因在妊娠大鼠卵巢中的表达,探讨RLF在大鼠妊娠中的作用。方法:采用RT-PCR方法。结果:妊娠第1天卵巢RLFmRNA高水平表达,妊娠第3天RLFmRNA水平最低,随着妊娠的进展,卵巢RLFmRNA的表达逐渐增加,至中期达到高水平,持续到妊娠后期。分娩前后RLFmRNA的水平出现波动,临近分娩RLFmRNA升高明显。结论:RLF参与妊娠的全过程。 Objective：To investigate the expression of RLFmRNA in the rat ovaries during pregnancy and study the effect of RLF in the process of pregnancy . Methods： Reverse transcriptase polymerase chain reaction （ RT - PCR） was used to study the expression of the RLFmRNA . Results：The first day when pregnancy occurred, RLFmRNA level was high, the third lowest level appeared, after that RLFmRNA expression to increase graduelly and reached high levels. In the porcess,continuing to the end of pregenancy, But fluctuated again when the rat labor at head. Conclusion： RLF is concerned with the whole process of pregnancy.
作者 张承玉 富泽龙 念红 金秀东
机构地区 牡丹江医学院生理教研室 牡丹江市肿瘤医院
出处 《牡丹江医学院学报》 2008年第1期1-2,共2页 Journal of Mudanjiang Medical University
基金 牡丹江医学院科研项目(B2006-08)
关键词 RLF 大鼠 卵巢 妊娠 RLF rat ovary pregnancy
分类号 R339.2 [医药卫生—人体生理学]
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参考文献6

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二级参考文献33

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共引文献8

同被引文献6

  • 1张承玉,赵薇,程丹玲,朱辉,金秀东.离体培养的大鼠卵巢颗粒细胞和黄体细胞中松弛素样因子mRNA的表达及调节[J].第二军医大学学报,2007,28(1):111-113. 被引量：4
  • 2Adham IM, Burkhardt E, Banahmed M, Engel W. Cloning of a cDNA for a novel insulin - like peptide of the testicular Leydig cells. J Biol Chem, 1993 Dec 15,268 (35) :26668 - 26672.
  • 3Nef S, Parada LF. Cryptorchidism in mice mutant for INSL3. Nat Genet, 1999,22:295 - 299.
  • 4Zimmermann S, Steding G, Emmen JM, et al.. Targeted disruption of the INSL3 gene causes bilateral cryptorchidism. Mol Endocfinol, 1999,13:681 - 691.
  • 5Irving - Rodgers HF, Bathgate RAD, Ivell R, et al. Dynamic changes in the expression of relaxin - like factor ( INSL3 ), cholesterol side - chain cleavage cytochrome P450, and 3.9 -hydroxysteroid dehydrogenase in bovine ovarian follicles during growth and atresia. Biol Reprod ,2002,66:934 - 943.
  • 6Boockfor FR, Fullbright G, Bullesbach EE, Schwabe C. Relaxinlike factor (RLF) serum concentrations and gubernacular RLF receptor display in relation to pre - and neonatal development of rats. Reproduction,2001 Dec,122(6) :899 -906.

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牡丹江医学院学报
2008年 第1期

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