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羟基脲诱导的K562细胞分化中PBK/TOPK的表达变化及其意义

Expression and Significance of PBK/TOPK Induced by Hydroxyurea During Differentiation of K562 Leukemia Cell
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摘要 目的研究PBK/TOPK在HU(羟基脲)诱导白血病细胞K562的分化时的表达及意义。方法用不同浓度的羟基脲对K562细胞进行诱导分化实验,通过联苯胺、吉姆萨-瑞氏染色和流式细胞仪证实其分化方向;用Western blot法检测PBK/TOPK在K562细胞分化前后表达的变化。结果400μmol/L的羟基脲对K562作用4d后具有良好的诱导分化效果,并诱导其向红系分化,且PBK/TOPK在K562细胞向成熟分化后表达不变,而Pho-PBK/TOPK和Pho-p38有少量增加。结论HU能抑制白血病细胞K562的增殖,并诱导其向红系分化,在此过程中,有激活的PBK/TOPK参与,推测很可能通过磷酸化下游分子p38而起作用。 Objective To investigate the effect of PBK/TOPK expression during differentiation of K562 leukemia. Methods The differentiation of K562 cells was induced by Hydroxyurea（HU） in different concentrations. The differentiation models were evaluated by the staining of benzidine and Wright-Giemsa and fluorescence activivated cell analyzer（FCAS）. Western blot was deteced the expression of PBK/TOPK. Results HU can induced elytroid differentiation of K562 cell line in the concentration of 400/μmol/L in 4 days. The expression of PBK/TOPK still unchanged, whereas phosphorylation PBK/TOPK and phosphorylation p38 were increased. Conclusion HU can inhibit the proliferation of K562 cells and induce the differentiation of K562 cells along the elytroid, phosphorylation PBK/TOPK expression play a role in this process by phosphorylation p38.
作者 刘宇宏 刘延香 王哲 黄高升 王文清 张伟平 王娟红 何芙蓉 李袁飞 王璐
机构地区 陕西延安大学附属医院血液科 第四军医大学基础部病理教研室
出处 《肿瘤防治研究》 CAS CSCD 北大核心 2008年第2期92-94,共3页 Cancer Research on Prevention and Treatment
关键词 K562细胞 PBK/TOPK 细胞分化 羟基脲 K562 cells PBK/TOPK Differentiation Hydroxyurea
分类号 R733.7 [医药卫生—肿瘤]
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参考文献8

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二级参考文献17

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肿瘤防治研究
2008年 第2期

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