蛋白酶活化受体-1在鼻咽癌中的表达及意义

Expression of Protease-Activated Receptor-1 in Nasopharyngeal Carcinoma and Its Significance

目的检测PAR1在鼻咽癌中的表达,探讨PAR1在鼻咽癌中表达的意义。方法免疫组织化学(SP法)检测28例良性鼻咽组织、61例鼻咽癌组织中PAR1中的表达;采用RT-PCR、Western Blot检测鼻咽癌细胞系CNE-1、CNE-2中PAR1表达并比较其表达差异。结果PAR1在鼻咽癌组织及两种鼻咽癌细胞株中均表达。PAR1在28例良性鼻咽组织表达为10例,61例鼻咽癌组织中表达48例。PAR1在鼻咽癌组织中的表达率明显高于在良性鼻咽组织中的表达率(P<0.01),高度恶性细胞株中PAR1的表达明显高于其低度恶性细胞株。结论PAR1的表达与鼻咽癌的发生、生长方式、分期、恶性程度等有关。PAR1在鼻咽癌中的表达可能介导鼻咽癌侵袭转移等恶性生物学行为。

Objective To investigate the expression of PAR1 in nasopharyngeal carcinoma （NPC）and its significance. Methods Immunohistochemical（SP method）was used to detect PAR1 expression in 28 cases of benign nasopharyngeal tissues and 61 cases of NPC. The relationship between the expression of PAR1 in NPC and the clinical parameters was retrospectively analyzed. Receptor expression on protein and mRNA level was evaluated in two human NPC cell lines （CNE-2 and CNE-1） by Western Blot, and RT-PCR. Results Of 61 cases of NPC examined, PAR1 expresses in 48 cases; of 28 cases of nasopharyngeal benign lesions examined, PAR1 expressed in 10 cases. The rate of PAR1 expressed in NPC was higher than that in the nasopharyngeal benign lesions （P〈0. 01 ）. The more malignant and metastasis cell line（CNE-2）expressed higher mRNA and protein level of PAR1 than the lowly malignant and metastasis cell line（CNE-1）by RT-PCR and Western Blot. Conclusion The expression of PAR1 is correlated with carcinogenesis, growth pattern, clinical staging, malignancy of NPC. PAR1 may contribute to the malignant phenotype and biological behavior in NPC.