摘要
目的探讨STAT3在喉癌组织中的表达及意义。方法采用免疫组织化学法和RT-PCR检测STAT3蛋白及mRNA在喉癌组织中的表达情况。结果免疫组织化学检测发现30例喉癌组织标本中有23例STAT3高表达,而8例正常组织中有2例低表达(P<0.05),6例未表达;RT-PCR检测STAT3mRNA的表达结果显示30例喉癌组织中21例呈高表达,相应的正常组织2例为低表达(P<0.05)。结论STAT3高表达于大多数喉癌组织中,较正常组织表达明显升高,提示STAT3的表达可能与喉癌的发生、发展密切相关。
Objective To study the expression of STAT3 in human carcinoma of larynx specimens. Methods The expression of STAT3 in human laryngeal carcinoma specimens was investigated by immunohistochemical staining. the expression of STAT3 mRNA in human laryngeal squamous cell carcinoma specimens was examined by using RT-PCR. Results Immunohistochemical staining analysis revealed that STAT3 was expressed in 23 of 30 human laryngeal carcinoma specimens but was expressed in 2 of 8 non- malignant laryngeal tissue（P〈0. 05）. The mRNA of STAT3 are positive in 21 of 30 human laryngeal carcinoma specimens but positive in 2 of 8 nonmalignant laryngeal specimens （P〈0. 05 ）. Conclusion Compared with nonmalignant laryngeal tissues, the expression level of STAT3 in laryngeal carcinoma tissues was significantly higher （P〈0. 05）. It suggests that the expression of STAT3 may be related to the risk and development of laryngeal carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第2期104-106,共3页
Cancer Research on Prevention and Treatment
基金
高等学校博士学科点专项科研基金资助项目(20020487062)
关键词
STAT3
喉肿瘤
基因表达
STAT3 Drotein
Laryngeal neoplasms
Gene expression
