Oct-4在不同侵袭转移能力的人胆管癌细胞系中的表达

Expression of Oct-4 in human cholangiocarcinoma cell lines with different metastatic capacities

目的探讨干细胞因子Oct-4在不同侵袭转移能力的人胆管癌QBC939亚克隆细胞系中的表达。方法运用体外侵袭实验筛选出1个高侵袭转移潜力和1个低侵袭转移潜力的细胞亚系,并对两者的生物学特性进行比较、分析和鉴定。运用RT-PCR,Western-blot的方法检测干细胞因子Oct-4在两个亚克隆细胞系的差异表达。结果分离得到高和低转移能力的人胆管癌QBC939亚克隆细胞系QBC-H和QBC-L,体外侵袭指数分别为263±5.9和154±6.6(P<0.05)。干细胞因子Oct-4 mR-NA和蛋白在QBC-H的表达均高于QBC-L,两者差异有显著性(P<0.05)。结论干细胞因子Oct-4的表达强度与胆管癌QBC939的侵袭转移能力密切相关;

Objective To investigate the expression of stem cell factor Oct-4 in human cholangiocarcinoma QBC939 subclone cell lines with different metastatic capacities. Methods The screening and use of one subclone cell line with high metastasis capacity and one subclone cell line with low metastasis capacity was performed by the in vitro invasive assay. Then, the biologic characters of the two subclone cell lines were compared, analyzed and identified. The expression of the stem cell factor Oct-4 in the two selected subclone cell lines was determined by RT-PCR and Western-blot assays. Results Two subclones of QBC939 with different metastatic capacities, and named QBC-H and QBC-L were isolated, and their invasion indexes were 263 ＋5.9 and 154 ＋6.6, respectively （P 〈0.05）. The expression of mRNA and protein of Oct-4 was stronger in QBC-H than in QBC-L. Conclusions There is a close relationship between the expression strength of the stem cell factor Oct-4 and the metastatic capacity of Oct-4. The stem cell factor may play an important role in regulation of the metastatic capacity of cholangiocareinoma.