载阿霉素聚氰基丙烯酸正丁酯纳米粒对L-02细胞的毒性研究

A study on cytotoxicity of ADM-PBCA-NP to L-02 cells

目的检验载阿霉素聚氰基丙烯酸正丁酯纳米粒(ADM-PBCA-NP)对人肝细胞株L-02细胞的毒性。方法体外培养人肝细胞株L-02,检测各组不同纳米粒浓度培养上清液中乳酸脱氢酶(LDH)的活性;用噻唑蓝(MTT)法检测L-02在与ADM-PBCA-NP,阿霉素(ADM)和空白纳米粒(PBCA-NP)共同培养的条件下对肝细胞的毒性,通过对不同浓度PBCA-NP的溶血试验测定其生物相容性。结果MTT结果显示,ADM组,ADM-NP组,PBCA-NP组在10-6mol/L的浓度范围内,细胞毒性均为1级,即对细胞无害。各组肝细胞L-02培养上清液中LDH活性的测定无统计学差异。结论在10-6mol/L浓度范围内,ADM-NP和PBCA-NP对肝细胞L-02无明显毒性作用;在一定的浓度范围内细胞相容性良好,不会引起溶血。

Objective To investigate the cytotoxicity of ADM-PBCA-NP on L-02 cells. Methods L-02 cells were cultured in vitro and the LDH activity of supernatant liquid of culture cells was examined. The toxicity of ADM-PBCA-NP, ADM and PBCA-NP to L-02 cells by the MTT assay was also determined, and the haemolysis function of PBCA-NP with different concentrations was detected. Results The cytotoxicity of ADM-PBCA-NP, ADM and blank PBCA nanoparticles to L-02 cells under the 10^-6 mol/L concentration range was not cytototic （grade 1 ）. LDH activity of supematant liquid of culture cells showed no differences between the 3 groups. Conclusions Nanoparticles of ADM-NP and PBCA-NP in the 10^-6 mol/L concentration range have no significant toxic effect on L-02 hepatic cells; and in a certain concentration range, the cell compatibility is excellent and will not lead to hemolysis.