Mycobacterium Phlei在黄铁矿和方铅矿表面的选择性吸附作用机理

Mechanism of selective adsorption of Mycobacterium Phlei on pyrite and galena surface

运用吸附量测定、ζ-电位研究、红外光谱和X射线光电子能谱分析,探讨Mycobacterium Phlei在黄铁矿和方铅矿表面的选择性吸附作用机理。结果表明,在其他实验条件固定的情况下,当溶液的初始pH值大于5时,Mycobacterium Phlei在黄铁矿表面的吸附量远远大于方铅矿;静电作用不是致使Mycobacterium Phlei在黄铁矿和方铅矿表面产生选择性吸附的主要作用力;Mycobacterium Phlei细胞表面的C、N和O元素通过矿物表面的Fe,Pb和S元素与矿物表面发生了化学吸附,而且与黄铁矿表面元素的作用程度大于方铅矿。矿物的表面结构及其性质、细胞表面基团与矿物表面金属离子的键合作用大小可能是致使Mycobacterium Phlei产生选择性吸附的主要原因。

By means of adsorption rate measurement, zeta-potential, infrared spectroscopy and X-ray photoelectron spectroscopy, the mechanism of selective adsorption of Mycobacterium Phlei on the surfaces of pyrite and galena was studied. The results show that Mycobacterium Phlei adsorbs preferentially on pyrite than on galena when pH is over 5 and other parameters are fixed, which is also observed by scanning electron microscopy. Static force is not the main function to make Mycobacterium Phlei has an obvious selective adsorption on the surfaces of pyrite and galena from the zeta potential. IR and XPS results indicate that chemical adsorption occurred on the surfaces of two minerals by Fe, Pb and S, and the reaction degree of elements C, N and O on the surface of Mycobacterium Phlei to surface elements of pyrite is larger than that of galena. It is concluded that the crystal frame and surface property of two minerals and the linking between groups on the surface of Mycobacterium Phlei and metal ions on the surface of two minerals are main factors that cause the selective adsorption of Mycobacterium Phlei.