硫酸壳聚糖体内抗肿瘤作用的实验研究

Experimental study on anti-tumor effect of chitosan sulfate in mice

目的:研究硫酸壳聚糖的体内抗肿瘤作用。方法:用高、低(200、100mg/kg)两个剂量的硫酸壳聚糖分别腹腔注射治疗肉瘤180(S180)小鼠和艾氏腹水癌(EAC)小鼠10d,然后测定其抑瘤率、重要器官的内脏指数和生命延长率,同时设生理盐水组(空白对照组)和氟尿嘧啶组(阳性对照组)进行比较。结果:硫酸壳聚糖高、低剂量组和氟尿嘧啶组的抑瘤率分别为38.67%、30.19%和43.27%,3组的生命延长率分别为65.38%、69.23%和54.93%。和生理盐水组、氟尿嘧啶组相比,硫酸壳聚糖高、低剂量组的S180小鼠的胸腺指数均有明显增加(P<0.05)。结论:硫酸壳聚糖能有效抑制S180小鼠肿瘤的生长和延长EAC小鼠的生存时间,其作用机制可能与其提高机体的免疫力有关。

AIM： To investigate the anti-tumor effects of chitosan sulfate in mice. METHODS： 80 mice were divided into control group, 5-FU treatment group and Chitosan sulfate treatment group （200 mg/kg and 100 mg/kg）. Different doses of Chitosan were intraperitoneally injected into mice with sarcoma 180 and EAC cancer respectively for 10 days. The tumor inhibiting rate, the weight index of important organ and life prolongation were calculated. RESULTS： The tumor inhibiting rate of high dose, low dose of Chitosan sulfate and 5-FU were 38.67%, 30.19%, 43.27%, life prolongation of high dose, low dose of Chitosan sulfate and 5-FU were 65.38%, 69.23%, 54.93%, Compare with control and 5-FU group, the indexes of thymus in high dose and low dose of Chitosan sulfate were significantly increased （ P 〈 0.05 ）. CONCLUTIONS： Chitosan sulfate shows significant anti-tumor effects on sareoma-180 and can prolong the life of mice with EAC cancer. Such effects may be owe to improvement of body immunity.