丹参颗粒剂对实验性缺血心肌的保护作用和对凝血系统的影响

Protective effects of Salvia przewalskii Maxim granule on experimental myocardial ischemia and coagulation system

目的:观察丹参颗粒剂对实验性缺血心肌模型的保护作用和对凝血系统的影响。方法:采用冠状动脉结扎法造成大鼠心肌缺血模型,心电图记录观察S-T段变化,测定心肌梗塞范围,测定血清乳酸脱氨酶(LDH)含量,测定大鼠心功能。用异丙肾上腺素诱导小鼠心肌缺血模型测定血清乳酸脱氢酶(LDH)和丙二醛(MDA)的含量。用langendorff氏法测定大鼠心脏冠脉流量。用胶原-肾上腺素混合液诱导小鼠体内血栓形成测定小鼠存活率。按Born比浊法,体外测定血小板最大聚集率。结果:丹参颗粒剂能明显减低缺血心肌S-T段的抬高,具有缩小大鼠心肌梗塞范围、改善大鼠缺血心肌的收缩功能、增加离体大鼠心脏冠脉流量的作用。能抑制缺血心肌中MDA含量升高和血清LDH的升高。明显提高诱导形成血栓小鼠的存活率及抗兔血小板聚集作用。结论:丹参颗粒剂对实验性缺血心肌具有保护和治疗作用,能抑制血小板聚集和对抗血栓形成。

Objective： To study the protective effects of Salvia przewalskii Maxim granule（SPG） on experimental myocardial ischemia and coagulation system. Methods： The acute myocardial ischemia model was established on rats by ligation of coronary artery ; meanwhile, the changes of S - T segment, myocardial infract size, contents of LDH and myocardial function were determined and assessed. The myocardial ischemia model was made by isoproterenol on mice and the contents of LDH and MDA were determined. The coronary flow of rats was observed by lan- gendorff＇s method. The survival rate of mice was observed after thrombus was formed induced by collagen and epinephrine. The platelet aggregation was determined by Born＇s method. Results： SPG can significantly reduce myocardial ischemia S -T segment elevation , the degree of myocardial ischemia and the myocardial infract size, decrease the contents of LDH and MDA of rats and mice, improve the contraction function of rats with myocardial ischemia and increase the flow of coronary. SPG has a significant inhibiting effect on the platelet aggregation of rabbits, and can improve the survival rate of mice which have formed thrombus induced by collagen and epinephrine. Conclusion：SPG has protective and therapeutic effects on the experiental myocardial ischemia of rats and mice, and it can inhibit platelet aggregation of rabbits and thrombus formation of rats.