米贝拉地尔对大鼠肠缺血再灌注损伤的影响

Effect of Mibefradil on Gut Injury Following Intestinal Ischemia Reperfusion in Rats

【目的】观察米贝拉地尔(Mibefradil)对大鼠肠缺血再灌注后肠组织损伤的影响。【方法】复制SD大鼠肠缺血再灌注损伤模型。实验分为3组(n=10):假手术组(S组)、缺血再灌注组(I/R组)、Mibefradil干预组(M组)。比较各组大鼠肠黏膜丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性变化;用Chiu氏评分法观察肠黏膜的损伤情况。【结果】与S、M组相比:I/R组MDA含量明显增高(P<0.01和P<0.05),SOD及CAT活性明显降低(P<0.01和P<0.05);与S组相比,I/R组和M组小肠损伤评分明显升高(P<0.01和P<0.05);但M组小肠损伤评分明显好于I/R组(P<0.05)。【结论】米贝拉地尔对大鼠肠缺血再灌注后的肠道有保护作用,该保护作用可能与减少活性氧物质及改善肠道微循环有关。

[Objective]To investigate the effect of Mibefradil on gut injury following intestinal ischemia reperfusion in rats. [Methods] By using rat model of intestine I/R injury, 30 male SD rats were randomly divided into 3 groups（ n = 10 in each group）： sham-operation group（S）, I/R group（I/R） and mibefradil group （M）. The contents of malondialdehyde （MDA）, the activity of superoxide dismutase （SOD） and catalase （CAT） in intestinal mucosa were measured respectively. Chiu＇s count was used to assess the changes in intestinal pathological morphology. [Results] In group I/R, MDA values were significantly increased compared to those in group S and M （ P 〈0.01 and P 〈0.05, respectively）. SOD and CAT values were significantly decreased in group I/R compared to group S and M （ P〈0.01 and P 〈0.05, respectively）. The intestinal injury score increased significantly in group I/R and M compared to group S （ P 〈0.01 and P 〈0.05, respectively）. However this increase was reduced in group M compared to group I/R （ P 〈0.05）. [Conclusion]Pretreatment with mibefradil has protective effect on intestinal ischemia-reperfusion injury in rats, which may be attributed to the decrease of reactive oxygen species （ROS） and restoration of intestinal microcirculatory blood flow.