血红素氧合酶-1在高血压病患者中的表达

Clinical study on changes of heme oxygenase-1 expression in patients with hypertension

目的观察血红素氧合酶-1(HO-1)与高血压患者血压的关系。方法选择确诊高血压病患者200例,分为:①低度危险组80例;②中度危险组50例;③高度危险组40例;④极高危险组30例;选择在读健康学生30名作为对照组。先利用t检验比较对照组与试验组HO-1表达的不同,再利用方差分析及Q检验比较4组高血压病患者HO-1表达的差异,找出HO-1与高血压病严重程度的关系。利用免疫组化方法和Western Blot检测高血压病患者外周血单个核细胞中HO-1的表达水平。结果高血压患者极高危险组HO-1表达明显高于高血压病患者低度危险组(P<0.01)。结论HO-1的表达水平与高血压病严重程度有关。

Objective To investigate the changes of heme oxygenase-1 in patients with hypertension. Methods The patients with hypertension （n=200） were divided into 4 groups：low degree danger group （n=80） medium degree danger group （n=50） high danger group（n=40） and very high danger group（n=30）,and control group （n=30）.The levels of HO-1 protein expression of monocyte and lymphocyte in patients were tested by immunohistochemistry and Western Blot. Results The level of HO-1 protein expression in patients with hypertension of very high danger were significantly higher than that in hypertension of low degree danger （P〈0.01）.Conclusion The level of HO-1 protein expression is associated with types of hypertension.