锌指转录因子Snail在结直肠癌中的表达及其意义

Expression of the zinc finger transcriptional factor Snail in colorectal carcinoma and its significance

目的确定锌指转录因子Snail与结直肠癌发生发展及演进的相关性。方法应用免疫细胞化学及免疫荧光细胞化学方法分析Snail在结直肠癌细胞株SW480与SW620内的表达情况,同时以结直肠癌组织68例及对应癌旁组织68例、腺瘤组织33例、淋巴结转移癌35例为研究对象,运用免疫组织化学方法分析Snail在这些组织中的表达情况。结果Snail在结直肠癌细胞株SW620、SW480中的表达较强,Snail定位在结直肠癌细胞株SW620、SW480的细胞核里;Snail在正常结直肠粘膜、腺瘤、癌及淋巴结转移癌组织中的表达明显不同(χ2=92.852,P=0.000);两两比较发现,腺瘤Snail表达比正常结直肠黏膜组织强(P<0.01),淋巴结转移灶中癌组织Snail表达比原发结直肠癌组织强(P<0.01),而原发结直肠癌组织Snail表达比腺瘤组织强(P<0.01)。结论锌指转录因子Snail与结直肠癌发生、演进及转移相关。

Objective To study expression of the zinc finger transcriptional factor Snail in colorectal carcinoma and its significance. Methods Expressions of Snail in colorectal carcinoma SW480 and SW620 cells were assayed by immunocytochemistry and immunofluorescent cytochemistry. The paraffin-embedded specimens from 68 cases of colorectal carcinoma and its corresponding adjacent tissues, 33 cases of adenoma and 35 cases of metastatic lymph nodes were also examinaed for Snail expressions using immunohistochemistry. Results Snail protein was located mainly in the cell nucleus of SW480 and SW620 cells. The expressions of PRL-3 protein in the specimens of colorectal carcinoma, normal mucosa, adenoma and metastatic lymph nodes were significantly different （~2 =92.852, P=0.000）. In the adenoma tissues, the expression was significantly higher than that in normal mucosa （Z=-2.902, P=0.004）, the metastatic lymphnodes had significantly higher expressions than the primary colorectal carcinomas （Z=-4.951, P=0.000）, which, in turn, showed significantly higher expression than the adenoma tissues （Z=-3.572, P=0.000）. Significant correlation of Snail expression was found to the progression and metastasis of colorectal carcinoma （Z=-2.043, P=0.041）. ConclusiOn The expression of Snail is significantly correlated to genesis, progression and metastasis of colorectal carcinoma.