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携带HBVC基因重组MVA假病毒颗粒的构建及其抗原性鉴定 被引量：1

Construction and antigenic evaluation of a recombinant MVA virus-like particle expressing HBV C gene
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摘要 目的构建携带HBVC基因的重组MVA假病毒颗粒并鉴定其抗原性。方法将HBVC基因通过基因重组构建入穿梭载体pSC11,得到质粒pSC11-C,将此质粒转染MVA病毒通过同源重组得到MVA-C。结果利用基因测序,PCR鉴定证实所得假病毒颗粒携带HBVC基因,并且具有良好的抗原性。经过9次传代得到单克隆重组病毒。结论成功构建了携带HBVC基因的重组MVA假病毒颗粒MVA-C,为研究HBV慢性感染的基因治疗提供了新途径。 Objective To construct the virus-like parcel expressing hepatitis B virus （HBV） C gene and identify its immtmogencity. Methods HBV C gene was cloned into the shuttle vector pSC11, and the resulted plasmid pSC11-C was transfected into modified vaccinia virus Ankara （MVA）. Results pSC11-C was correctly constructed as verified by sequence analysis and PCR, and the recombinant virus-like parcel possessed good immtmogencity. Coneluslon The MVA-C expressing HBV C gene has been successfully constructed to provide important basis for gene therapy research of chronic HBV infection.
作者 栾翔凌 孔维 刘素军 雷厉 胡燕 侯俊 沈宏辉 吴贻琛 游绍莉 貌盼勇 辛绍杰
机构地区 中国人民解放军军医进修学院 吉林大学 北京朝阳医院京西院区 中国人民解放军
出处 《南方医科大学学报》 CAS CSCD 北大核心 2008年第2期252-254,共3页 Journal of Southern Medical University
关键词 痘苗病毒 肝炎病毒 乙型 基因治疗 vaccina virus hepatitis B virus gene therapy
分类号 R392 [医药卫生—免疫学]
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参考文献10

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共引文献967

同被引文献10

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南方医科大学学报
2008年 第2期

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